Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily. In this case, instead of the molecular ion [M + HI' (m/z 414), the base peak corresponds to the [M + NHJ' ion (m/z 431) and again the fragment of the electrophilic moiety is also present. Their physicochemical characteristics, subcellular prediction, and cis-acting elements were investigated. Comprehensive expression analysis suggests overlapping and specific roles of rice glutathione S-transferase genes during development and stress responses. XW, XL, Y-qA, HZ, DM, YJ, and HH analyzed the data. 1), is the most important low molecular weight antioxidant synthesized in cells.It is synthesized by the sequential addition of cysteine to glutamate followed by the addition of glycine. Genome-wide identification and functional characterization Biol. Plant J. Overexpression of RcGPX4 in Arabidopsis decreased sensitivity to cold stress at the germination stage but showed hypersensitivity to freezing stress at seedlings. (2013). Z., Zhang, X. Y., Gong, Z. Table 2. The anti-ferroptotic function of FSP1 was found to be independent of cellular glutathione levels, GPX4 activity, ACSL4 expression and oxidizable fatty acid content (Extended Data Figs. Glutathione S-transferases (GSTs) compose a family of multifunctional enzymes that play important roles in the detoxification of xenobiotics and the oxidative stress response. Plant Sci. It is imperative to reveal the molecular mechanism underlying the resistance of castor beans to inferior growth conditions. Putative cis-acting regulatory elements associated with stress and hormone responses in RcGPX promoters. (2017). Are you trying to find Glutathione for sale? The different colors of each branch indicated the different groups. A role for thiol-peptides in the long-distance transport of cadmium and the effect of cadmium on iron translocation. J. Mol. Sci. Front. The freezing tolerance assay was performed using 14-day-old seedlings, which were pre-treated at 4C for 3 days, exposed to 10C for 5 h, thawed at 4C for 12 h, and then recovered at 22C for 5 days before counting survival rate. Identification of glutathione transferase gene associated with partial resistance to Sclerotinia stem rot of soybean using genome-wide association and linkage mapping Theor Appl Genet. Plant GPXs carry cysteine residue in the active site and have a preference for Trx as electron donors (Navrot et al., 2006; Koua et al., 2009). Genome-wide association mapping of Sclerotinia sclerotiorum resistance in soybean using whole-genome resequencing data. Can J Plant Sci 66:559564 Plant J. Guan, C., Wang, X., Feng, J., Hong, S., Liang, Y., Ren, B., et al. Because mitochondria are the major consumers of molecular oxygen wi Detecting sequence signals in targeting peptides using deep learning. The survival rate of WT was about 80% whereas that of OE lines was between 40 and 52%, which suggested that RcGPX4 negatively mediated seedlings of transgenic plants in response to freezing treatment (Figure 6). doi: 10.1007/s00299-020-02512-4, Yoshimura, K., Miyao, K., Gaber, A., Takeda, T., Kanaboshi, H., Miyasaka, H., et al. Bot. Glutathione (GSH) is an endogenous tripeptide composed of the amino acids L-glutamate, L-cysteine, and glycine. It has a role as a skin lightening agent, a human metabolite, an Escherichia coli metabolite, a mouse metabolite, an antioxidant and a cofactor. Int. The identification of the transporter may lead to a better understanding of a variety of disease pathways linked to oxidative stress, including those involved in aging and neurodegeneration. Asterisks indicate a significant difference between wild type and overexpressed lines (P < 0.05, P < 0.01, students t-test). Plant J. RT: room temperature (22C); CT-1: cold temperature (4C) for 1 day; CT-2: cold temperature (4C) for 2 days. (2000). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. Herein, a total of five GPXs were identified from the current R. communis genome. Huelsenbeck, J. P., Ronquist, F., and Hall, B. In animals engineered to lack this protein, red blood cells quickly die by oxidative stress due to their failure to bring glutathione into mitochondria. Request PDF | Engineering the Redox-Driven Channel for Precisely Regulating Nanoconfined Glutathione Identification and Transport | Bioinspired artificial nanochannels for molecular and ionic . The improved seed germination in transgenic lines was associated with the alteration of ABA/GA/CTK pathway-related genes under cold stress. Bioresource Technol. Glutathione peroxidase of Pennisetum glaucum (PgGPx) is a functional Cd2+ dependent peroxiredoxin that enhances tolerance against salinity and drought stress. -, Bastien M, Huynh TT, Giroux G, Iquira E, Belzile F (2012) A reproducible assay for measuring partial resistance to Sclerotinia sclerotiorum in soybean. A putative lambda class glutathione S-transferase enhances plant survival under salinity stress. To analyze the properties of the five RcGPXs, the RcGPXs were aligned to identify the conserved residues within the families from the castor bean, cucumber, Arabidopsis, and rice using ClustalW. Genome-wide analysis of glutathione S-transferase gene family in chickpea suggests its role during seed development and abiotic stress. Please enable it to take advantage of the complete set of features! A comparison of phylogenetic relationships, protein motifs, and gene structures revealed that the structure and function of most GPXs may have been conserved in diverse plant species during evolution. Extraction characterization and modification of castor seed oil. Molecular characterization of two glutathione peroxidase genes of Panax ginseng and their expression analysis against environmental stresses. 275:3959. doi: 10.1111/j.1742-4658.2008.06542.x, Miao, Y. C., Lv, D., Wang, P. C., Wang, X. C., Chen, J., Miao, C., et al. Copyright 2021 Wang, Liu, An, Zhang, Meng, Jin, Huo, Yu and Zhang. However, a systematic analysis of GST family members in Brassica napus has not yet been reported. These results revealed a significant degree of conservation of the GPXs family in the exon-intron arrangement in diverse plant species. (2001). The deduced molecular weights (MWs) and PIs of the five RcGPXs were 18.8129.48 kDa and 4.979.20, respectively. However, the transcriptional level of ICE1, CBFs, and downstream CORs were lower in OE lines than in WT under cold stress. He Y, Mao S, Gao Y, Zhu L, Wu D, Cui Y, Li J, Qian W. PLoS One.

doi: 10.1199/tab.0131. (2015). Reactive oxygen species abiotic stress and stress combination.

doi: 10.1093/molbev/msn067, Lescot, M., Dehais, P., Thijs, G., Marchal, K., Moreau, Y., Van de Peer, Y., et al. To further explore the molecular mechanism of the reduced freezing tolerance regulated by RcGPX4 in transgenic seedlings, we investigated the expression level of cold-responsive genes involved in CBF-dependent and ABA-dependent pathways. The region used for generating the phylogenetic tree was obtained by excluding sites with alignment gaps (where gaps were found in over 5% of each alignment site) and missing data, leaving 166 positions in the final dataset. doi: 10.1104/pp.113.234740, Horton, P., Park, K. J., Obayashi, T., Fujita, N., Harada, H., Adams-Collier, C. J., et al. Interestingly, motif 4 was specific to AtGPX1, AtGPX6, and RcGPX3. Genome-wide identification and expression analysis of the glutathione S-transferase (GST) family under different developmental tissues and abiotic stresses in Chinese cabbage (Brassica rapa ssp . Both types of reaction result in the formation of a mixed . The transgenic plants showed more accumulation of MDA compared with WT under cold stress, which suggested a higher degree of lipid peroxidation in transgenic plants (Figure 7B). A growing body of evidence indicated that GPXs can protect the cells from damage caused by abiotic stresses (Roxas et al., 2000; Islam et al., 2015b; Zhang et al., 2019).

Zhao X, Han Y, Li Y, Liu D, Sun M, Zhao Y, Lv C, Li D, Yang Z, Huang L, Teng W, Qiu L, Zheng H, Li W. Plant J. (A) H2O2 content; (B) MDA content; (C) SOD activity; (D) POD activity; (E) CAT activity; (F) APX activity; (G) reduced GSH content; (H) GSSG content; (I) GSH/GSSG ratio; (J) GR activity; and (K) DHAR activity. Rep. 7:44743. doi: 10.1038/srep44743, Chinnusamy, V., Ohta, M., Kanrar, S., Lee, B. H., Hong, X., Agarwal, M., et al. A body of studies suggested that plant GPXs acted as crucial regulatory roles in response to diverse environmental factors. 51 - 60 Article Download PDF View Record in Scopus Google Scholar Comparative Analysis of the Glutathione S-Transferase Gene Family of Four. Boudhrioua C, Bastien M, Torkamaneh D, Belzile F. BMC Plant Biol. Previous studies in model and ornamental plants indicated members of the glutathione S-transferase (GST) gene family were involved in vacuolar accumulation of anthocyanins. doi: 10.1016/j.jmb.2007.04.031, Koua, D., Cerutti, L., Falquet, L., Sigrist, C. J. Total 1,500 bp sequences upstream from the translational start site of each gene were surveyed to predict cis-acting regulatory elements against the PlantCare database (Lescot et al., 2002). Duplicated and orthologous genes in Brassica . doi: 10.1093/nar/gkm259, Hu, B., Jin, J., Guo, A. Y., Zhang, H., Luo, J., and Gao, G. (2015). The freezing tolerance assay was done using 14-day-old seedlings. doi: 10.1101/gad.1077503, Choudhury, F. K., Rivero, R. M., Blumwald, E., and Mittler, R. (2017).

Cross talk between H2O2 and interacting signal molecules under plant stress response. 2020 Jul 15;747:144674. doi: 10.1016/j.gene.2020.144674. 176, 192201. Healthy male and female human volunteers were exposed to 50 ppm or 100 ppm trichloroethylene (Tri) by inhalation for 4 h. In the present study, twenty four GST genes from the transcriptome of a metal-tolerant dark septate endophyte (DSE), Exophiala pisciphila, were identified based on sequence homology, and their responses to various heavy . Functional analysis of oxidative stress-acti-vated mitogen-activated protein kinase cascade in plants. Structural Insights into Omega-Class Glutathione Transferases: A Snapshot of Enzyme Reduction and Identification of a Non-Catalytic Ligandin Site Joseph Brock1, Philip G. Board2, Aaron J. Oakley3* 1 Research School of Chemistry, Australian National University, Canberra, Australian Capital Territory, Australia, 2 John Curtin School of Medical Research, Australian National University, Canberra . Found inside Page 322Beutler , E. and West , C. , Comment concerning a fluorometric assay for glutathione , Anal . Biochem . , 81 , 458 , 1977 . 10. Ball , C. R. , Estimation and identification of thiols in rat spleen after cysteine or glutathione treatment In this study, the expression level of MPK3 was significantly higher in OE plants compared to WT in both conditions. Additionally, RcGPX4 depended on the MAPK3-ICE1-C-repeat-binding factor (CBF)-COR signal transduction pathway and ABA-dependent pathway to negatively regulate the freezing tolerance of transgenic plants. Arabidopsis chloroplastic glutathione peroxidases play a role in cross talk between photooxidative stress and immune responses. Effect of different stresses on the expression of the RcGPXs in leaves, roots, and stems. Several abiotic stresses and hormone-responsive cis-acting elements existed in the promoters of the RcGPXs. Two wheat GPXs were overexpressed in Arabidopsis and displayed higher salt and H2O2 tolerance compared to wild type (WT) (Zhai et al., 2013). Search for more papers by this author. The Bayesian method was constructed from thirteen 13 plant species, including such as 75 GPX homologs to explore their phylogenetic relationship (Figure 3). Plant glutathione peroxidases are functional peroxiredoxins distributed in several subcellular compartments and regulated during biotic and abiotic stresses. THE JOURNAL OF BIOLOGICAL CHEMISTRY 0 1988 by The American Society for Biochemistry and Molecular Biology, Inc. Vol. Author links open overlay panel Jian Gao a b Baowei Chen a Haijian Lin c Yi Liu c Yao Wei a Fabo Chen a b Wenbo Li a b. Found inside Page 202From a more practical perspective , the current class nomenclature for cytoplasmic glutathione transferases is most useful when classes are limited to proteins that share more than 5060 % identity . The -mu , -alpha , and -pi classes Identification of a cis-2-butene-1,4-dial-derived 2010;8:e0131.

Accumulating evidence pointing to mitochondria as critical participants in the control of apoptotic and necrotic cell death and in the development of specific disease states has led to a renaissance on the study of these organelles. doi: 10.1006/meth.2001.1262, Margis, R., Dunand, C., Teixeira, F. K., and Margis-Pinheiro, M. (2008). The plant has evolved important physiological mechanisms, such as the GSH-ascorbate pathway, to cope with the damage caused by excessive H2O2. A total of 330 TaGST genes were identified from the wheat genome and named according to the . The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity. Glutathione S-transferases (GSTs) is a family of multifunctional enzymes that are involved in detoxification of poisonous compounds. doi: 10.1371/journal.pone.0143344, Jana, G. J., and Yaish, M. W. (2020). Mol. Identification of the major soluble cuticular glycoprotein of lymphatic filarial nematode parasites (gp29) as a secretory homolog of glutathione peroxidase. Plant Sci. Glutathione transferases (GSTs) are dimeric enzymes containing one active-site per monomer. GPXs play an essential role in plant growth and development by participating in photosynthesis, respiration, and stress tolerance. Alliance 2:e201900429. Identification and comparative analysis of H2O2-scavenging enzymes (ascorbate peroxidase and glutathione peroxidase) in selected plants employing bioinformatics approaches Front. In our study, we surprisingly found that OE lines had higher H2O2 contents as compared to WT, which was not consistent with previous findings. The content of H2O2 was significantly higher in transgenic lines than in WT under normal and cold stress conditions (Figure 7A). (2015).

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